ZNF354C knockout or knockdown increased its target gene expression in HMECs, and ZNF354C with mutations in box A of its KRAB domain exhibited dominant-negative effects on endothelial angiogenic sprouting.Kid3 audio tag editor 3.8.6 was released a few days ago with new features and important bug-fixes. The KRAB domain of ZNF354C interacted with double-stranded DNA, TRIM28 (601742), and histone-3 (H20) and directly bound to target gene promoters. Transcriptional repression by ZNF354C was mediated by the conserved MLE sequence within box A of its KRAB domain. (2020) showed that ZNF354C inhibited angiogenic sprouting of cultured HMECs, HUVECs, and HAoSMCs, as ZNF354C acted as a direct transcriptional repressor of many genes. ZNF354C knockout or knockdown increased its target gene expression in HMECs, and ZNF354C with mutations in box A of its KRAB domain exhibited dominant-negative effects on endothelial angiogenic sprouting.īy overexpression analysis, Oo et al. The KRAB domain of ZNF354C interacted with double-stranded DNA, TRIM28 ( 601742), and histone-3 (H20) and directly bound to target gene promoters. The CCAC core sequence in DNA was essential for KID3 binding through its zinc finger domain.īy overexpression analysis, Oo et al. Mutation analysis demonstrated that KID3 was a sequence-specific transcription factor that bound to DNA. (2004) showed that KID3 was a transcription repressor, with the KRAB domain functioning as a transcription repressor domain. Using a luciferase reporter gene, Gao et al. Immunofluorescence analysis revealed that ZNF354C localized to nucleus and cytoplasm, with accumulation at the nuclear membrane. (2020) showed that ZNF354C was expressed in all human tissues tested, with strong expression in vascular cells, such as human umbilical vein endothelial cells (HUVECs), human microvascular endothelial cells (HMECs), and human aortic smooth muscle cells (HAoSMCs). Using RT-PCR and Western blot analyses, Oo et al. Immunofluorescence analysis showed that fluorescence-tagged human KID3 localized to nuclei of transfected COS7 cells, and the zinc finger domain was both necessary and sufficient for nuclear localization. The 8.5-kb transcript was not detected in adult tissues. The 6-kb transcript was also expressed in human adult skeletal muscle and weakly in other adult tissues, such as brain and heart. Northern blot analysis detected transcripts of 6 and 8.5 kb predominantly in human fetal brain and kidney. A 134-amino acid spacer region is present between the KRAB domain and the first zinc finger. The deduced 554-amino acid human KID3 protein has an N-terminal KRAB domain and 11 tandemly repeated C2H2 zinc finger motifs at the C terminus. (2004) cloned KID3 from a human embryo cDNA library. Expression of Kid3 in kidney was developmentally regulated. Northern blot analysis showed that Kid3 was highly expressed in embryonic and adult mouse brain, with lower levels in adult and embryonic kidney, gut, lung and heart. The deduced 560-amino acid protein has an N-terminal KRAB transcriptional repression domain and 11 C2H2 zinc fingers. (2000) cloned mouse Znf354c, which they called Kid3.
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